Chindoko Antibody Rapid Test kaseti
Syphilis TP Antibody Rapid Test Kits Diagnostic Kit
CHIDULE
Njira yodziwira matenda ndi TP imagwiritsidwa ntchito pozindikira kuti ali ndi matenda a Syphilis (TP) m'magazi athunthu a munthu, seramu kapena plasma.Njira ya Colloidal Goldndipo akhoza kupereka zotsatira mkati mwa mphindi 15.
ZOYENERA KUGWIRITSA NTCHITO
Mayeso a One Step TP ndi Golide wa Colloidal wokwezedwa,.Zachipatala, mankhwalawa amagwiritsidwa ntchito makamaka pozindikira matenda a Treponema pallidum.Izi ndizogwiritsidwa ntchito ndi azachipatala okha.
MFUNDO YA NJIRA
Mankhwalawa amatengera mfundo ya sangweji ya antigen iwiri.Pamene chitsanzocho chili ndi antibody ya Treponema pallidum, antibody ya Treponema pallidum mu chitsanzocho imakhudzidwa ndi golide wa colloidal otchedwa-syphilis recombinant antigen 1 papepala lomangiriza kuti apange antigen-antibody complex.Vutoli limapangidwa ndi chromato-graphed kutsogolo kudzera mu capillary action ndipo limagwidwa ndi recombinant syphilis antigen 2 yomwe imakutidwa pa detecticn area(T line) ya nembanemba ya nitrocellulose, ndipo gulu lofiira limawonekera.Chomeracho chikupitilira kupangidwa ndi chromatograph chokwera, ndipo cholembera cha golide cha lgY colloidal chimatengedwa ndi anti-chicken lgY antibody yomwe idakutidwa pamalo owongolera (C line) ya membrane ya nitrocellulose, ndipo gulu lofiira limawonekera.Pamene zomwe zili mu analyte mu chitsanzo ndizochepa kusiyana ndi malire odziwika bwino, malo odziwika (T mzere) sapanga mtundu.
NTCHITO YAIKULU
1.Test PAD, payekha mmatumba mu thumba zotayidwa zojambulazo (1piece / thumba, 1/5/10/25/50chidutswa (s) / kit)
2. Udzu wa pulasitiki wotayika (1 picce / thumba, 1/5/10/25/50 chidutswa (s) / zida)
3.Chikwama cha zinyalala zachipatala(1picce/thumba,1/5/10/25/50 chidutswa(s)/kit)
4. Buku la malangizo (1kope/thumba, 1 kope/kit)
Zindikirani: Zomwe zili mumagulu a manambala osiyanasiyana sasinthana.
ZIMENE ZIMENE UNGACHITE
Kusakaniza kwachitsanzo (chidutswa chimodzi/chikwama,1/5/10/25/50 chidutswa/zidutswa)
Padi ya mowa wa thonje(chidutswa/thumba,1/5/10/25/50 chidutswa(zi)/kiti)
口 singano yosonkhanitsira magazi(chidutswa chimodzi/chikwama,1/5/10/25/50 chidutswa/zidutswa)
ZINTHU ZOFUNIKA KOMA ZOSAPATSIDWA
Zowongolera zabwino ndi zoyipa (zopezeka ngati chinthu chapadera)
KUSINTHA NDI KUKHALA
Zoyikapo zoyambirira ziyenera kusungidwa pamalo ouma pa 4-30 ℃ otetezedwa ku kuwala, ndipo musawume.
CHENJEZO NDI CHENJEZO
Zidazi ndi zokhazikika mkati mwa tsiku lotha ntchito lomwe lidasindikizidwa.Choyesacho chiyenera kugwiritsidwa ntchito mkati mwa l ola pansi pa 4-30 ℃ ndi chinyezi <65% chikwama cha aluminiyamu chikatsegulidwa.Ndibwino kuti mugwiritse ntchito nthawi yomweyo kutentha kwambiri kapena kutentha kwambiri.
KUSONKHA ZITSANZO NDI KUSINTHA
2. Kusungirako zitsanzo
2.1 Mwazi wonse;Machubu a Anticoagulant amagwiritsidwa ntchito posonkhanitsa magazi, komanso wambaanticoagulants angagwiritsidwe ntchito;ngati magazi athunthu sangathe kugwiritsidwa ntchito nthawi yomweyozosonkhanitsira, zitha kusungidwa pa 2-8 ° C kwa masiku atatu, ndipo zitsanzo sizingawumitsidwe.
2.2 Seramu / plasma: Zitsanzo zitha kusungidwa pa 2-8 ℃ kwa masiku 7, ndipo ziyenera kukhalazosungidwa pa -20 ℃ kuti zisungidwe kwa nthawi yayitali.
3.Only sanali hemolyzed zitsanzo ayenera kugwiritsidwa ntchito.Severely hemolyzed zitsanzo ayeneraayesedwenso.
4 Zitsanzo za firiji ziyenera kubwezeretsedwanso ku kutentha kwa chipinda chisanayesedwe.TheZitsanzo zachisanu ziyenera kusungunuka kwathunthu, kutenthedwa, ndi kusakaniza mofanana kalentchito.Osaundana ndi kusungunuka mobwerezabwereza
NJIRA YOYENERA
1) Pogwiritsa ntchito chotsitsa chapulasitiki chotsekeredwa pazachitsanzo, perekani dontho limodzi (10μl) la Magazi Onse / Seramu / Plasma pachitsime chozungulira cha khadi yoyesera.
2) Onjezani madontho a 2 a Sample Diluent pachitsime, nthawi yomweyo chitsanzocho chikawonjezedwa, kuchokera padontho la diluent vial (kapena zonse zomwe zili mu ampule imodzi yoyeserera).
3) Tanthauzirani zotsatira za mayeso pa mphindi 15.
Ndemanga:
1) Kugwiritsa ntchito kuchuluka kokwanira kwa diluent ndikofunikira kuti mupeze zotsatira zolondola.Ngati kusamuka (kunyowetsa kwa nembanemba) sikukuwoneka pazenera loyesa pakatha mphindi imodzi, onjezerani dontho lina la diluent pachitsimecho.
2) Zotsatira zabwino zitha kuwoneka posachedwa mphindi imodzi yachitsanzo chokhala ndi ma antibodies apamwamba a TP.
3) Osamasulira zotsatira pakatha mphindi 20
KUWERENGA ZOTSATIRA ZA MAYESERO
1)Zabwino: Mayeso onse a purplish red test band ndi purplish red control bandi zimawonekera pa nembanemba.Kutsika kwa ma antibodies kumachepa, gulu loyesera limacheperachepera.
2) Zoipa: Ndi gulu lowongolera lofiira la purplish lokha lomwe limawonekera pa nembanemba.Kusowa kwa gulu loyesera kukuwonetsa zotsatira zoyipa.
3)Zotsatira zosalondola:Nthawi zonse payenera kukhala gulu lowongolera lofiira la purplish m'chigawo chowongolera, mosasamala kanthu za zotsatira za mayeso.Ngati gulu lowongolera silikuwoneka, mayesowo amawonedwa ngati osavomerezeka.Bwerezani kuyesa pogwiritsa ntchito chipangizo choyesera chatsopano.
Zindikirani: Ndi zachilendo kukhala ndi chowongolera chopepuka pang'ono chokhala ndi zitsanzo zolimba kwambiri, bola ziwonekere bwino.
MALIRE
1) Magazi Oyera / Seramu / Plasma okha angagwiritsidwe ntchito poyesa izi.
2) Zitsanzo zatsopano ndizabwino koma zitsanzo zachisanu zitha kugwiritsidwa ntchito.Ngati chitsanzo chazizira, chiyenera kuloledwa kuti chisungunuke pamalo oyima ndikuwonetsetsa ngati madzi ali ndi madzi.Magazi Athunthu sangaumitsidwe.
3) Osasokoneza chitsanzo.Ikani pipette pansi pa pamwamba pa chitsanzo kuti mutenge Chitsanzo.
