Ofufuza achi Dutch amaphatikiza CRISPR ndi bioluminescence mu mayeso oyeseramatenda opatsirana

Mapuloteni omwe angopangidwa kumene usiku amatha kufulumizitsa komanso kupeputsa matenda obwera chifukwa cha ma virus, malinga ndi ofufuza ku Netherlands.
Kafukufuku wawo, wofalitsidwa Lachitatu mu ACS Publications, akufotokoza njira yowonongeka, imodzi yokha yowunikira mofulumira ma nucleic acids ndi maonekedwe awo pogwiritsa ntchito mapuloteni owala a buluu kapena obiriwira.
Kuzindikiritsa tizilombo toyambitsa matenda pozindikira zala zawo za nucleic acid ndi njira yofunika kwambiri pakuwunika zachipatala, kafukufuku wazachipatala, komanso kuyang'anira chitetezo chazakudya ndi chilengedwe.Mayesero omwe amagwiritsidwa ntchito kwambiri a polymerase chain reaction (PCR) ndi ovuta kwambiri, koma amafunikira kukonzekera kwachitsanzo kapena kutanthauzira kwazotsatira, zomwe zimapangitsa kuti zikhale zovuta pazochitika zina zachipatala kapena zoikamo zochepa.
Gulu ili lochokera ku Netherlands ndilo zotsatira za mgwirizano pakati pa asayansi ochokera ku mayunivesite ndi zipatala kuti apange njira yofulumira, yonyamula komanso yosavuta kugwiritsa ntchito nucleic acid yomwe ingagwiritsidwe ntchito pazochitika zosiyanasiyana.
Anauziridwa ndi kuwala kwa ziphaniphani, kuwala kwa ziphaniphani, ndi nyenyezi zazing'ono za phytoplankton zam'madzi, zonse zoyendetsedwa ndi chodabwitsa chotchedwa bioluminescence.Kuwala-mu-mdima kumeneku kumadza chifukwa cha kachitidwe ka mankhwala okhudza mapuloteni a luciferase.Asayansi adaphatikiza mapuloteni a luciferase m'masensa omwe amatulutsa kuwala kuti athe kuwona akapeza chandamale.Ngakhale izi zimapangitsa kuti masensa awa akhale abwino kuti adziwike, pakali pano alibe chidwi chachikulu chofunikira pakuyezetsa matenda.Ngakhale njira yosinthira jini ya CRISPR imatha kupereka izi, imafunikira masitepe ambiri ndi zida zina zapadera kuti zizindikire chizindikiro chofooka chomwe chingakhalepo mu zitsanzo zovuta, zaphokoso.
Ochita kafukufuku apeza njira yophatikizira mapuloteni okhudzana ndi CRISPR ndi chizindikiro cha bioluminescent chomwe chingathe kudziwika ndi kamera ya digito yosavuta.Kuti atsimikizire kuti pali RNA kapena DNA yokwanira yowunikira, ofufuzawo adachita recombinase polymerase amplification (RPA), njira yosavuta yomwe imagwira ntchito kutentha kosalekeza kwa 100 ° F.Anapanga nsanja yatsopano yotchedwa Luminescent Nucleic Acid Sensor (LUNAS), momwe mapuloteni awiri a CRISPR/Cas9 ndi odziwika bwino a magawo osiyanasiyana amtundu wa ma virus, iliyonse ili ndi chidutswa chapadera cha luciferase chomwe chili pamwambapa.
Pamene majeremusi enieni a ma virus omwe ofufuza akufufuza alipo, mapuloteni awiri a CRISPR / Cas9 amamangiriza ku ndondomeko ya nucleic acid;amakhala moyandikana kwambiri, kulola kuti mapuloteni a luciferase osasunthika apange ndikutulutsa kuwala kwa buluu pamaso pa gawo la mankhwala..Kuwerengera gawo lapansi lomwe limagwiritsidwa ntchito pochita izi, ofufuzawo adagwiritsa ntchito njira yowongolera yomwe idatulutsa kuwala kobiriwira.Chubu chomwe chimasintha mtundu kuchokera ku wobiriwira kupita ku buluu chimasonyeza zotsatira zabwino.
Ofufuzawo adayesa nsanja yawo ndikupanga RPA-LUNAS assay, yomwe imazindikiraSARS-CoV-2 RNApopanda kudzipatula kwa RNA yotopetsa, ndikuwonetsa momwe amawonera pamiyeso ya nasopharyngeal swab kuchokeraCOVID 19odwala.RPA-LUNAS idazindikira bwino SARS-CoV-2 mkati mwa mphindi 20 mu zitsanzo zokhala ndi ma virus a RNA otsika mpaka makope 200/μL.
Ofufuzawo amakhulupirira kuti kuyesa kwawo kumatha kuzindikira mosavuta komanso moyenera ma virus ena ambiri."RPA-LUNAS ndiyokongola pakuyezetsa matenda opatsirana," adalemba.