Chiyambireni mliriwu, kuyezetsa matenda kwatenga gawo lofunikira pakuwongolera kufalikira kwa SARS-CoV-2, kachilombo komwe kamayambitsa.COVID 19.Mayeso othamanga a antigenzochitidwa kunyumba kapena kuchipatala zimapereka zotsatira mu mphindi 15 kapena kuchepera.Munthu akapezeka msanga, m’pamene angakapeze chithandizo chamankhwala mwamsanga n’kudzipatula kwa ena.Koma mitundu yatsopano ya kachilomboka ikawonekera, zosinthazo sizingadziwike ndi mayesowa.
Mayeso othamanga kwambiri a antigen adapangidwa kuti azindikire mapuloteni a SARS-CoV-2 nucleocapsid kapena N-protein.Puloteni imeneyi imapezeka mochulukira mu tinthu ta tizilombo toyambitsa matenda komanso anthu amene ali ndi kachilomboka.Rapid test kitNthawi zambiri amakhala ndi ma antibodies awiri osiyana omwe amamanga mbali zosiyanasiyana za N protein.Antibody ikamangirira ku puloteni ya N mu zitsanzo, mzere wamitundu kapena chizindikiro china chimawonekera pa zida zoyesera, kuwonetsa matenda.
Mapuloteni N ali ndi 419 amino acid mayunitsi.Aliyense wa iwo akhoza m'malo ndi ena amino asidi ndi masinthidwe.Gulu lofufuza motsogozedwa ndi Ph.D.Philip Frank ndi Eric Ortlund a ku yunivesite ya Emory anayamba kufufuza momwe kusintha kumodzi kwa amino acid kumakhudzira ntchito ya kuyesa kwa antigen mofulumira.Adagwiritsa ntchito njira yotchedwa deep mutation scanning kuti awone nthawi imodzi momwe kusintha kulikonse mu puloteni ya N ya kachilomboka kumakhudzira kumangirira kwa anti-antibody.Zotsatira zawo zidasindikizidwa mu Cell pa Seputembara 15, 2022.
Ofufuzawo adapanga laibulale yokwanira pafupifupi 8,000 N zosinthika zama protein.Zosinthazi zimapitilira 99.5% ya masinthidwe onse omwe angathe.Kenako adawunika momwe mtundu uliwonse umalumikizirana ndi ma antibodies 17 osiyanasiyana omwe amagwiritsidwa ntchito pamayeso 11 opezeka mwachangu a antigen, kuphatikiza wamba.zida zapanyumba.
Gululo lidayesa kusintha kwa ma N-protein komwe kumakhudza kuzindikira kwa antibody.Kutengera chidziwitsochi, adapanga "mbiri yopulumukira" pamtundu uliwonse wa antibody.Mbiriyi imazindikiritsa masinthidwe enieni a mapuloteni a N omwe angakhudze kuthekera kwa antibody kumangirira ku cholinga chake.Kuwunikaku kunawonetsa kuti ma antibodies omwe amagwiritsidwa ntchito poyesa mwachangu masiku ano amazindikira ndikumanga mitundu yonse yakale komanso yamakono ya SARS-CoV-2 yodetsa nkhawa komanso yodetsa nkhawa.
Ngakhale ma antibodies angapo amazindikira gawo lomwelo la mapuloteni a N, ofufuzawo adapeza kuti antibody iliyonse ili ndi siginecha yapadera yosinthira kuthawa.Pamene kachilombo ka SARS-CoV-2 kakupitilira kusinthika ndikupanga mitundu yatsopano, izi zitha kugwiritsidwa ntchito kuyika ma antibodies omwe angafunikire kuwunikiridwanso.
"Kuzindikiritsa kolondola komanso koyenera kwa anthu omwe ali ndi kachilombo kumakhalabe njira yofunika kwambiri yochepetsera COVID-19, ndipo kafukufuku wathu amapereka chidziwitso pakusintha kwamtsogolo kwa SARS-CoV-2 komwe kungasokoneze kuzindikira," adatero Ortlund."Zotsatira zomwe tafotokozazi zikutilola kuti tizolowere kachilomboka mwachangu pomwe mitundu yatsopano ikupitilira, zomwe zikuwonetsa zovuta zachipatala komanso zaumoyo."
Zoyambira: Mutation Deep Scan imazindikira masinthidwe othawa mu SARS-CoV-2 nucleocapsid pogwiritsa ntchito mayeso othamanga a antigen omwe alipo.Frank F., Kin MM, Rao A., Bassit L., Liu H, Bowers HB, Patel AB, Kato ML, Sullivan JA, Greenleaf M., Piantadosi A., Lam VA, Hudson VH, Ortlund EA cell.2022 September 15;185(19):3603-3616.e13.Utumiki Wamkati: 10.1016/j.cell.2022.08.010.Ogasiti 29, 2022 PMID: 36084631.
Ndalama: National Institute for Biomedical Imaging and Bioengineering NIH (NIBIB), National Institute of Diabetes, Digestive and Kidney Diseases (NIDDK) ndi National Institute of Allergy and Infectious Diseases (NIAID), American Heart Association.
NIH Research Matters ndikusintha kwa sabata pazotsatira zazikulu za NIH zowunikiridwa ndi akatswiri a NIH.Imasindikizidwa ndi Ofesi ya Communications and Public Affairs ya Director of the National Institutes of Health.