page

mankhwala

STEPI YAMODZI HCV KUYESA (Magazi Onse / Seramu / Madzi a m'magazi)

Kufotokozera Kwachidule:


Mankhwala Mwatsatanetsatane

FAQ

Zogulitsa

STEPI YAMODZI HCV KUYESA (Magazi Onse / Seramu / Madzi a m'magazi)

hcv rna
anti hcv test
hcv antibody
hcv test
hepatitis c test

Chidule

Njira yodziwira matenda a HCV ndikuwona kupezeka kwa ma antibodies ku kachilomboka ndi njira ya EIA yotsatiridwa ndi Western Blot. Kuyesa kwa Gawo Limodzi ndi HCV ndiyeso losavuta, lowoneka bwino lomwe limazindikira ma antibodies m'mwazi Wathu Wonse wa magazi / seramu / plasma. Kuyesaku kutengera immunochromatography ndipo kumatha kupereka zotsatira mkati mwa mphindi 15.

ZOFUNIKA KUGWIRITSA NTCHITO

The One Step HCV Test ndi Colloidal Gold yolimbikitsidwa, yofulumira ya Immunochromatoraphic Assay pakuzindikira koyenera kwa ma antibodies ku Hepatitis C Virus (HCV) mu Human Whole Blood / Serum / Plasma. Kuyesaku ndikoyesa kuwunika ndipo zabwino zonse ziyenera kutsimikiziridwa pogwiritsa ntchito mayeso ena monga Western Blot. Mayesowa adapangidwira kuti azigwiritsa ntchito Healthcare Professional kokha. Kuyezetsa konse ndi zotsatira za kuyesedwaku zikuyenera kuti zingagwiritsidwe ntchito ndi akatswiri azachipatala ndi azamalamulo okha, pokhapokha ngati zololedwa mwalamulo mdziko logwiritsa ntchito. Kuyesaku sikuyenera kugwiritsidwa ntchito popanda kuyang'aniridwa koyenera.

MFUNDO ZA M'NTHAWIYI

Kuyesaku kumayamba ndi nyemba zomwe zagwiritsidwa ntchito pachitsanzo bwino ndikuwonjezeranso zomwe zaperekedwa nthawi yomweyo. HCV antigen-Colloidal Gold conjugate yomwe ili mu phukusi loyeserera limagwira ndi anti-HCV antibody yomwe ilipo mu seramu kapena plasma, ndikupanga ma conjugate / HCV antibody complex. Pamene kusakanikako kumaloledwa kusunthira pamzere woyeserera, makina a conjugate / HCV amatengedwa ndi puloteni yolimbana ndi anti-A yomwe imasunthika pamiyendo yopanga gulu lachikuda mdera loyeserera. Zoyipa sizitulutsa mzere woyeserera chifukwa chakusowa kwa ma colloidal Gold conjugate / HCV antibody complex. Ma antigen omwe amagwiritsidwa ntchito poyeserera ndi mapuloteni ophatikizidwanso omwe amafanana ndi zigawo za HCV zopanda chitetezo. Gulu lowongolera lachikuda m'chigawo chowongolera limapezeka kumapeto kwa njira yoyeserera mosasamala zotsatira zake. Gulu lowongolera ili ndi zotsatira za Colloidal Gold conjugate yomangiriza kwa anti-HCV antibody yokhazikika pamimbayo. Mzere wowongolera ukuwonetsa kuti Colloidal Gold conjugate imagwira ntchito. Kusakhala kwa gulu lowongolera kumawonetsa kuti mayeserowa ndi osavomerezeka.

REAGENTS NDI ZOTHANDIZA ZOPEREKEDWA

Chipangizo choyesera payekha zojambulidwa ndi phukusi la desiccant

• Choponyera pulasitiki.

• Zitsanzo Zovuta

• phukusi Ikani

ZIPANGIZO ZOFUNIKA KOMA ZISAPEREKEDWE

Kuwongolera koyenera komanso koyipa (komwe kumapezeka ngati chinthu chosiyana)

YOSUNGA & kukhazikika

Makiti oyeserera ayenera kusungidwa pa 2-30 ℃ mu thumba losindikizidwa komanso pansi pouma.

CHENJEZO NDI CHENJEZO

1) Zotsatira zonse zabwino ziyenera kutsimikiziridwa ndi njira ina.

2) Chitani zitsanzo zonse ngati kuti zingakhale zopatsirana. Valani magolovesi ndi zovala zoteteza mukamagwiritsa ntchito zitsanzo.

3) Zipangizo zomwe zimagwiritsidwa ntchito poyesa ziyenera kujambulidwa zisanachitike.

4) Musagwiritse ntchito zida zopitilira masiku awo atha ntchito.

5) Osasinthana ma reagents m'malo osiyanasiyana.

KUSANTHULA KWA SAMPLE NDI KUSUNGA

1) Sonkhanitsani zitsanzo zonse za Magazi / Seramu / Plasma kutsatira njira zanthawi zonse za labotale.

2) Kusungirako: Magazi athunthu sangakhale oundana. Choyimira chimayenera kukhala mufiriji ngati sichinagwiritsidwe ntchito tsiku lomwelo losonkhanitsa. Mitundu iyenera kuzizira ngati isagwiritsidwe ntchito pasanathe masiku atatu asonkhanitsidwa. Pewani kuzizira ndikuchotsa zojambulazo nthawi zopitilira 2-3 musanagwiritse ntchito. 0.1% ya Sodium Azide itha kuwonjezeredwa kuchitsanzo ngati chosungitsa osakhudza zotsatira za kuyesa.

MFUNDO ZOTHANDIZA

1) Pogwiritsa ntchito choponya pulasitiki chotsekeracho, perekani 1 dontho (10μl) ya Magazi Onse / Seramu / Plasma kuzitsanzo zozungulira za khadi loyeserera

2) Onjezerani madontho awiri a Sampuli Yoyeserera pachitsanzo bwino, chitangowonjezeredwa, kuchokera pachitsime chothira madzi (kapena zonse zomwe zili mumayeso amodzi).

3) Tanthauzirani zotsatira zamayeso mphindi 15. 

310

Zolemba:

1) Kugwiritsa ntchito zitsanzo zokwanira zokwanira kuti pakhale mayeso olondola. Ngati kusunthira (kunyowa kwa nembanemba) sikukuwonetsedwa pazenera loyesera pakadutsa mphindi imodzi, onjezerani dontho limodzi la mankhwalawa pachitsimecho.

2) Zotsatira zabwino zitha kuoneka mphindi imodzi yokha pamayeso omwe ali ndi ma antibodies ambiri a HCV.

3) Osatanthauzira zotsatira pakadutsa mphindi 20

KUWERENGA ZOTSATIRA ZA KUYESA

1) Zabwino: Onse gulu loyesera loyera komanso loyera lofiira limapezeka pakhungu. Kutsika kwa chitetezo cha antibody, kumafooketsa gulu loyeserera.

2) Zoipa: Ndi gulu lokha loyera lomwe limatuluka pakhungu. Kusapezeka kwa gulu loyeserera kukuwonetsa zotsatira zoyipa.

3) Chotsatira cholakwika: Nthawi zonse payenera kukhala gulu loyang'anira lofiira lofiirira mdera loyang'anira, mosatengera zotsatira zoyeserera. Ngati gulu lowongolera siliwonekere, mayeso amayesedwa osagwira. Bwerezani kuyesaku pogwiritsa ntchito chida chatsopano choyesera.

Chidziwitso: Sizachilendo kukhala ndi gulu lowongolera pang'ono lokhala ndi zitsanzo zabwino kwambiri, bola ngati limawoneka bwino.

MALIRE

1) Ndi magazi omveka bwino okha, atsopano, komanso otuluka mwaulere Mwazi / Seramu / Plasma omwe angagwiritsidwe ntchito poyesaku.

2) Zatsopano ndizabwino koma zitsanzo zachisanu zitha kugwiritsidwa ntchito. Ngati nyemba yasungunuka, ziyenera kuloledwa kusunthira ndikuwunika ngati zili bwino. Magazi Onse Sangakhale oundana.

3) Osasokoneza chitsanzocho. Ikani pipette pansipa pamunsi pa nyemba kuti mutolere mtunduwo. 


  • Previous: Zamgululi
  • Ena:

  • Lembani uthenga wanu apa ndi kutumiza kwa ife