Gawo limodzi la mayeso a HCV Kaseti (Magazi Onse / Seramu / Plasma)
CHOYESA CHIMODZI CHA HCV (Magazi Onse/Seramu/Magazi a m'magazi)
CHIDULE
Njira yodziwira matenda ndi HCV ndikuwona kukhalapo kwa ma antibodies ku kachilomboka pogwiritsa ntchito njira ya EIA yotsatiridwa ndi kutsimikizira ndi Western Blot.Mayeso a One Step HCV ndi mayeso osavuta, owoneka bwino omwe amazindikira ma antibodies mu Magazi Onse / seramu / plasma.Mayesowa amatengera immunochromatography ndipo amatha kupereka zotsatira mkati mwa mphindi 15.
ZOYENERA KUGWIRITSA NTCHITO
The One Step HCV Test ndi Colloidal Gold yowonjezeredwa, Immunochromatoraphic Assay yofulumira pozindikira ma antibodies ku Hepatitis C Virus (HCV) mu Human Whole Blood / Serum / Plasma.Mayesowa ndi mayeso owunika ndipo zabwino zonse ziyenera kutsimikiziridwa pogwiritsa ntchito mayeso ena monga Western Blot.Mayesowa amapangidwa kuti azingogwiritsidwa ntchito ndi Healthcare Professional.Zonse zoyezetsa ndi zotsatira za kuyezetsazo zimapangidwira kuti zizigwiritsidwa ntchito ndi akatswiri azachipatala ndi zamalamulo okha, pokhapokha atavomerezedwa ndi malamulo m'dziko lomwe akugwiritsidwa ntchito.Mayesowa asagwiritsidwe ntchito popanda kuyang'aniridwa moyenera.
MFUNDO YA NJIRA
Kuyesa kumayamba ndi chitsanzo chomwe chimagwiritsidwa ntchito pachitsime chachitsanzo ndikuwonjezeranso sampuli yomwe yaperekedwa nthawi yomweyo.HCV antigen-Colloidal Gold conjugate yomwe ili mu pad yachitsanzo imakhudzidwa ndi anti-HCV antibody yomwe ilipo mu seramu kapena plasma, kupanga conjugate/HCV antibody complex.Pamene kusakaniza kumaloledwa kusuntha motsatira mzere woyesera, anti-conjugate/HCV antibody complex imagwidwa ndi puloteni yomangira ma antibody A osasunthika pa nembanemba kupanga gulu lachikuda m'chigawo choyesera.Chitsanzo choyipa sichimapanga mzere woyesera chifukwa chosowa Colloidal Gold conjugate/HCV antibody complex.Ma antigen omwe amagwiritsidwa ntchito poyesa ndi mapuloteni ophatikizananso omwe amafanana ndi zigawo za HCV zomwe zili ndi chitetezo chokwanira kwambiri.Gulu lolamulira lachikuda m'dera lolamulira likuwonekera kumapeto kwa njira yoyesera mosasamala kanthu za zotsatira zoyesa.Gulu lowongolera ili ndi zotsatira za Colloidal Gold conjugate yomanga ku anti-HCV anti-HCV osasunthika pa nembanemba.Mzere wowongolera ukuwonetsa kuti Colloidal Gold conjugate imagwira ntchito.Kusowa kwa gulu lowongolera kukuwonetsa kuti mayesowo ndi olakwika.
REAGENTS NDI Zipangizo ZOPEREKA
Chida choyesera chojambula pachokha choyikidwa ndi desiccant
• Chotsitsa chapulasitiki.
• Chitsanzo cha Diluent
• phukusi Ikani
ZINTHU ZOFUNIKA KOMA ZOSAPATSIDWA
Zowongolera zabwino ndi zoyipa (zopezeka ngati chinthu chapadera)
KUSINTHA NDI KUKHALA
Zida zoyesera ziyenera kusungidwa pa 2-30 ℃ mu thumba lomata komanso pansi pauma.
CHENJEZO NDI CHENJEZO
1) Zotsatira zabwino zonse ziyenera kutsimikiziridwa ndi njira ina.
2) Chitani zitsanzo zonse ngati zitha kupatsirana.Valani magolovesi ndi zovala zodzitetezera pogwira zitsanzo.
3) Zipangizo zomwe zimagwiritsidwa ntchito poyesa ziyenera kukhala autoclaved musanatayidwe.
4) Osagwiritsa ntchito zida zomwe zidapitilira masiku awo otha ntchito.
5) Osasinthanitsa ma reagents osiyanasiyana.
KUSONKHA ZITSANZO NDI KUSINTHA
1) Sonkhanitsani zitsanzo za Magazi Onse / Seramu / Plasma potsatira njira za labotale zachipatala.
2) Kusungirako: Mwazi Wonse sungathe kuzizira.Chitsanzo chiyenera kusungidwa mufiriji ngati sichikugwiritsidwa ntchito tsiku lomwelo la kusonkhanitsa.Zitsanzo ziyenera kusungidwa ngati sizikugwiritsidwa ntchito mkati mwa masiku atatu mutatolera.Pewani kuzizira ndi kusungunula zitsanzozo kuposa nthawi 2-3 musanagwiritse ntchito.0.1% ya Sodium Azide ikhoza kuwonjezeredwa ku chitsanzo ngati chosungira popanda kusokoneza zotsatira za kuyesa.
NJIRA YOYENERA
1) Pogwiritsa ntchito chotsitsa chapulasitiki chotsekeredwa pazachitsanzo, perekani dontho limodzi (10μl) la Magazi Onse / Seramu / Plasma pachitsime chozungulira cha khadi yoyesera.
2) Onjezani madontho a 2 a Sample Diluent pachitsime, nthawi yomweyo chitsanzocho chikawonjezedwa, kuchokera padontho la diluent vial (kapena zonse zomwe zili mu ampule imodzi yoyeserera).
3) Tanthauzirani zotsatira za mayeso pa mphindi 15.
Ndemanga:
1) Kugwiritsa ntchito kuchuluka kokwanira kwa diluent ndikofunikira kuti mupeze zotsatira zolondola.Ngati kusamuka (kunyowetsa kwa nembanemba) sikukuwoneka pazenera loyesa pakatha mphindi imodzi, onjezerani dontho lina la diluent pachitsimecho.
2) Zotsatira zabwino zitha kuwonekera pakangopita mphindi imodzi yachitsanzo chokhala ndi ma antibodies ambiri a HCV.
3) Osamasulira zotsatira pakatha mphindi 20
KUWERENGA ZOTSATIRA ZA MAYESERO
1)Zabwino: Mayeso onse a purplish red test band ndi purplish red control bandi zimawonekera pa nembanemba.Kutsika kwa ma antibodies kumachepa, gulu loyesera limacheperachepera.
2) Zoipa: Ndi gulu lowongolera lofiira la purplish lokha lomwe limawonekera pa nembanemba.Kusowa kwa gulu loyesera kukuwonetsa zotsatira zoyipa.
3)Zotsatira zosalondola:Nthawi zonse payenera kukhala gulu lowongolera lofiira la purplish m'chigawo chowongolera, mosasamala kanthu za zotsatira za mayeso.Ngati gulu lowongolera silikuwoneka, mayesowo amawonedwa ngati osavomerezeka.Bwerezani kuyesa pogwiritsa ntchito chipangizo choyesera chatsopano.
Zindikirani: Ndi zachilendo kukhala ndi chowongolera chopepuka pang'ono chokhala ndi zitsanzo zolimba kwambiri, bola ziwonekere bwino.
MALIRE
1) Magazi Oyera / Seramu / Plasma okha angagwiritsidwe ntchito poyesa izi.
2) Zitsanzo zatsopano ndizabwino koma zitsanzo zachisanu zitha kugwiritsidwa ntchito.Ngati chitsanzo chazizira, chiyenera kuloledwa kuti chisungunuke pamalo oyima ndikuwonetsetsa ngati madzi ali ndi madzi.Magazi Athunthu sangaumitsidwe.
3) Osasokoneza chitsanzo.Ikani pipette pansi pa pamwamba pa chitsanzo kuti mutenge Chitsanzo.
