Cholinga Chogwiritsidwa Ntchito

Mayeso a Dengue NS1 Ag-IgG/IgM Combo ndi kuyesa kwachangu kwa chromatographic immunoassay kwa qualitatively ya ma antibodies (IgG ndi IgM) ndi kachilombo ka dengue NS1 antigen ku virus ya dengue mu Serum kapena Plasma kuthandiza kuzindikira matenda a Dengue virus.

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Dengue fever ndi matenda opatsirana omwe amayamba chifukwa cha kachilombo ka dengue komwe kamafala ndi udzudzu.Matenda a dengue amatha kuyambitsa matenda ochulukirapo, dengue fever, dengue hemorrhagic fever, dengue hemorrhagic fever.Zizindikiro zodziwika bwino za malungo a dengue zimaphatikizapo kuyambika kwadzidzidzi, kutentha thupi, kupweteka mutu, kupweteka kwambiri kwa mafupa, mafupa ndi mafupa, totupa pakhungu, kutulutsa magazi, kukulitsa kwa lymph node, kuchepa kwa maselo oyera a magazi, thrombocytopenia ndi zina zotero.Matendawa ali m'madera otentha komanso otentha kwambiri, chifukwa matendawa amafalitsidwa ndi udzudzu wa Aides, chifukwa kutchuka kumakhala ndi nyengo, kukhala chaka chilichonse mu May ~ November, pachimake ndi July ~ September.M'dera latsopanolo la mliri, anthu ambiri amatha kutenga kachilomboka, koma nthawi zambiri amakhala akuluakulu, m'dera lomwe lili ndi mliriwu, nthawi zambiri amakhala ana.

Mfundo yofunika

Mayeso a Dengue NS1 Ag-IgG/IgM Combo ndi njira yoyesera yodziwira matenda a dengue virus (IgG ndi IgM) ndi antigen ya dengue NS1 mu Seramu kapena Plasma.

Kwa Mayeso a IgG/IgM : Chipangizo choyesera chimakhala ndi: 1) cholembera chamtundu wa burgundy chokhala ndi ma antigen a dengue ophatikizana ndi golide wa Colloid (ma conjugates a dengue), 2) chingwe cha nitrocellulose chokhala ndi mizere iwiri yoyesera (T1 ndi T2 mizere) ndi mzere wowongolera (C line).Mzere wa T1 umayikidwa kale ndi anti-dengue kuti azindikire IgM anti-dengue, mzere wa T2 umakutidwa ndi anti-dengue kuti azindikire IgG anti-dengue.Pamene chiwerengero chokwanira cha chitsanzo choyesera chaperekedwa mu chitsime cha chitsanzo cha kaseti yoyesera, chitsanzocho chimasuntha ndi capillary action kudutsa kaseti.IgG anti-dengue, ngati ilipo pachitsanzocho, imamanga ma conjugates a dengue.The immunocomplex ndiye anagwidwa ndi reagent chisanadze TACHIMATA pa T2 gulu, kupanga burgundy wachikuda T2 mzere, kusonyeza dengue IgG HIV zotsatira ndi kutanthauza matenda posachedwapa kapena kubwereza.IgM anti-dengue ngati ilipo pachitsanzo ichi imalumikizana ndi ma conjugates a dengue.The immunocomplex kenako anagwidwa ndi reagent yokutidwa pa T1 mzere, kupanga burgundy mtundu T1 mzere, kusonyeza dengue IgM HIV HIV zotsatira ndi kusonyeza matenda atsopano.Kusakhalapo kwa mizere ya T (T1 ndi T2) kukuwonetsa zotsatira zoyipa.

Pa Mayeso a NS1: Pakuyesa uku, anti-Dengue NS1 antibody imakhala yosasunthika mugawo loyesa la makaseti.Chitsanzo cha Magazi Athunthu / Seramu / Plasma chikayikidwa pachitsanzocho, chimakhudzidwa ndi tinthu tating'ono ta anti-Dengue NS1 antibody tomwe tayikidwa pa pepala lachitsanzo.Kusakaniza kumeneku kumasuntha motengera kutalika kwa mzere woyeserera ndikulumikizana ndi anti-Dengue NS1 antibody.Ngati chitsanzocho chili ndi kachilombo ka dengue NS1 antigen, mzere wachikuda udzawonekera m'chigawo cha mzere wosonyeza zotsatira zabwino.Ngati chithunzichi chilibe kachilombo ka dengue NS1 antigen, mzere wachikuda suwoneka m'chigawochi wowonetsa zotsatira zoyipa.

Kuti ikhale yoyang'anira njira, mzere wachikuda udzawonekera nthawi zonse pagawo la mzere wosonyeza kuti chiwerengero choyenera cha chitsanzo chawonjezeredwa ndipo kupukuta kwa membrane kwachitika.

Kusungirako ndi Kukhazikika

Sungani monga mmatumba mu thumba losindikizidwa kutentha firiji kapena firiji (4-30 ℃ kapena 40-86 ℉).Chipangizo choyesera ndi chokhazikika mpaka tsiku lotha ntchito lomwe lasindikizidwa pathumba losindikizidwa.

Mayeso amayenera kukhala muthumba losindikizidwa mpaka atagwiritsidwa ntchito.

Kusonkhanitsa ndi Kukonzekera Zitsanzo

1. Mayeso a Dengue NS1 Ag-IgG/IgM Combo atha kugwiritsidwa ntchito pa Serum kapena Plasma.

2. Kutenga magazi athunthu, seramu kapena madzi a m'magazi potsatira njira za labotale zachipatala.

3. Kuyezetsa kumayenera kuchitidwa mwamsanga pambuyo posonkhanitsa zitsanzo.Osasiya zitsanzo pa kutentha kwapakati kwa nthawi yayitali.Kusungirako nthawi yayitali, zitsanzo ziyenera kusungidwa pansi pa 20 ℃.

4. Bweretsani zitsanzo ku kutentha kwa chipinda musanayese.Zitsanzo zozizira ziyenera kusungunuka kwathunthu ndikusakaniza bwino musanayese.Zitsanzo siziyenera kuzizira ndi kusungunuka mobwerezabwereza.

Njira Yoyesera

Lolani kuyesa, chitsanzo, buffer ndi/kapena zowongolera kuti zifikire kutentha kwapakati pa 15-30 ℃ (59-86 ℉) musanayesedwe.

1. Bweretsani thumbalo kuti lizizizira bwino musanatsegule.Chotsani chipangizo choyesera m'thumba lomata ndikuchigwiritsa ntchito posachedwa.Ikani chipangizo choyesera pamalo oyera komanso osalala.

2. Pa Mayeso a IgG/IgM :Gwirani chotsitsa molunjika ndi kusamutsa dontho limodzi la chitsanzo (pafupifupi 10μl) kuchitsanzo chabwino(S) cha chipangizo choyesera, kenaka yikani madontho awiri a buffer (pafupifupi 70μl) ndikuyamba chowerengera.Onani chithunzi pansipa.

3. Pakuti NS1 Mayeso: Gwirani dropper vertically ndi kusamutsa 8 ~ 10 madontho a seramu kapena madzi a m'magazi (pafupifupi 100μl) kwa chitsanzo bwino (S) chipangizo mayeso, ndiye yambani chowerengera.Onani chithunzi pansipa.

4. Dikirani kuti mizere yachikuda iwonekere.Werengani zotsatira pa mphindi khumi ndi zisanu.Osamasulira zotsatira pakatha mphindi 20.

Kutanthauzira Zotsatira

Zabwino:

Kwa Mayeso a IgG/IgM: Mzere wowongolera ndi mzere umodzi woyesera umawoneka pa nembanemba.Maonekedwe a mzere woyesera wa T2 akuwonetsa kukhalapo kwa ma antibodies a dengue enieni a IgG.Maonekedwe a mzere woyeserera wa T1 akuwonetsa kukhalapo kwa ma antibodies a dengue enieni a IgM.Ndipo ngati mzere wa T1 ndi T2 ukuwonekera, zikuwonetsa kukhalapo kwa ma antibodies a dengue enieni a IgG ndi IgM.Kutsika kwa ma antibodies kumachepa, mzere wotsatira umachepa.

Kwa Mayeso a NS1: Mizere iwiri ikuwonekera.Mzere umodzi uyenera kuwonekera nthawi zonse m'chigawo cha mzere wowongolera (C), ndipo mzere wina wowoneka bwino uyenera kuwonekera m'chigawo cha mzere woyeserera.

Zoipa:

Mzere umodzi wachikuda umapezeka m'chigawo cholamulira (C). Palibe mzere wowoneka bwino wamitundu womwe umapezeka m'chigawo cha mzere woyesera.

Zosavomerezeka: Mzere wowongolera ukulephera kuwonekera.Kusakwanira kwa kuchuluka kwa zitsanzo kapena njira zolakwika ndizo zifukwa zomwe zimalepheretsa mzere wowongolera.Unikaninso ndondomekoyi ndikubwereza kuyesanso ndi chida chatsopano choyesera.Vuto likapitilira, siyani kugwiritsa ntchito zida zoyeserera nthawi yomweyo ndipo funsani wofalitsa wapafupipafupi.