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COVID-19 Neutralizing Antibody Rapid Test Cassette (Golide wa Colloidal)

Kufotokozera Kwachidule:


Tsatanetsatane wa Zamalonda

Zolemba Zamalonda

title

Kaseti ya COVID-19 Neutralizing Antibody Rapid Test (Colloidal Gold) ndi kuyesa kwachangu kwa chromatographic pakuzindikiritsa kwamphamvu kwa ma antibodies ku COVID-19 m'magazi amunthu, seramu, kapena plasma monga chothandizira pakuzindikiritsa kupezeka kwa ma antibodies. ku COVID-19.

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Ma coronaviruses atsopano ndi amtundu wa β. COVID-19 ndi matenda opatsirana pachimake kupuma. Nthawi zambiri anthu amavutika. Pakadali pano, odwala omwe ali ndi kachilombo ka coronavirus ndiye gwero lalikulu la matenda; anthu omwe ali ndi matenda asymptomatic amathanso kukhala gwero lopatsirana. Malingana ndi kafukufuku wamakono wa epidemiological, nthawi yoyamwitsa ndi masiku 1 mpaka 14, makamaka masiku 3 mpaka 7. The mawonetseredwe aakulu monga malungo, kutopa ndi youma chifuwa. Kutsekeka kwa mphuno, mphuno, zilonda zapakhosi, myalgia ndi kutsekula m'mimba zimapezeka nthawi zingapo.

Kaseti ya COVID-19 Neutralizing Antibody Rapid Test (Colloidal Gold) ndi kuyesa kofulumira komwe kumagwiritsa ntchito tinthu tambiri ta S-RBD antigen tozindikira kuti ma antibodies osalowerera ndale ku COVID-19 m'magazi amunthu, seramu, kapena plasma.

title2

Kaseti ya COVID-19 Neutralizing Antibody Rapid Test (Colloidal Gold) ndi membala woyeserera wodziwikiratu kuti ma antibodies osalowerera ndale ku COVID-19 m'magazi athunthu, seramu kapena plasma. Nembanembayo idakutidwa kale ndi Angiotensin I Converting Enzyme 2 (ACE2) pagawo loyesa la mzerewo. Poyesedwa, magazi onse, seramu kapena madzi a m'magazi amakhudzidwa ndi S-RBD conjugated colloid golide. Kusakaniza kumasunthira pamwamba pa nembanemba chromatographically ndi capillary kanthu kuti igwirizane ndi ACE2 pa nembanemba ndikupanga mzere wamitundu. Kukhalapo kwa mzere wamtundu uwu kumasonyeza zotsatira zoipa, pamene kusowa kwake kumasonyeza zotsatira zabwino. Kuti ikhale yoyang'anira ndondomeko, mzere wamtundu udzasintha nthawi zonse kuchokera ku Buluu kupita ku Red mu gawo la mzere wolamulira, kusonyeza kuti chiwerengero choyenera cha chitsanzo chawonjezeredwa ndipo kupukuta kwa membrane kwachitika.

title3
Zida zoyesera zodzaza payekhapayekha Chida chilichonse chimakhala ndi mzere wokhala ndi ma conjugates achikuda ndi ma reagents okhazikika omwe amafalitsidwa kale m'magawo ofanana.
Ma pipette otayika Kuwonjezera zitsanzo ntchito
Bafa Phosphate saline wothira mchere komanso woteteza
Ikani phukusi Pakuti malangizo ntchito
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Zida Zoperekedwa

●Zida zoyesera ●Madontho
●Bafa   ● Ikani phukusi

Zinthu Zofunika Koma Zosaperekedwa

● Zotengera zosonkhanitsira zitsanzo ● Nthawi
●Chipinda chapakati  
title5

1. Kwa akatswiri mu vitro diagnostic ntchito kokha.
2. Musagwiritse ntchito pambuyo pa tsiku lotha ntchito lomwe lasonyezedwa pa phukusi. Osagwiritsa ntchito mayeso ngati thumba la zojambulazo lawonongeka. Osagwiritsanso ntchito mayeso.
3. The m'zigawo reagent njira lili ndi mchere njira ngati njira kukhudzana khungu kapena diso, kusungunula ndi mvula yambiri madzi.

4. Pewani kuipitsidwa ndi tizitsanzo pogwiritsira ntchito chidebe chatsopano chosonkhanitsira chitsanzo pa chitsanzo chilichonse chomwe mwapeza.
5. Werengani ndondomeko yonse mosamala musanayesedwe.
6. Osadya, kumwa kapena kusuta m'malo omwe zitsanzo ndi zida zapakhomo zimasungidwa. Gwirani zitsanzo zonse ngati zili ndi tizilombo toyambitsa matenda. Yang'anirani njira zodzitetezera polimbana ndi zoopsa za tizilombo toyambitsa matenda munthawi yonseyi ndikutsata njira zokhazikika zotayira bwino zitsanzo. Valani zovala zodzitchinjiriza monga makhoti a mu labotale, magolovesi otayika komanso zoteteza maso mukayesedwa.
7. Ngati akuganiziridwa kuti ali ndi kachilombo koyambitsa matenda a coronavirus akuganiziridwa potengera momwe akuyezera matenda komanso miliri yomwe akuluakulu aboma akuvomereza, zitsanzo ziyenera kusonkhanitsidwa ndi njira zoyenera zopewera matenda a coronavirus yatsopano ndikutumizidwa ku dipatimenti ya zaumoyo m'boma kapena kwanuko kuti akayezedwe. Chikhalidwe cha ma virus sichiyenera kuyesedwa pamilandu iyi pokhapokha ngati BSL 3+ ilipo kuti mulandire ndi zikhalidwe.
8. Osasinthana kapena kusakaniza ma reagents osiyanasiyana.
9. Chinyezi ndi kutentha kungawononge zotsatira zake.
10. Zida zoyesera zogwiritsidwa ntchito ziyenera kutayidwa malinga ndi malamulo a m'deralo.

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1. Zida ziyenera kusungidwa pa 2-30 ° C mpaka tsiku lotha ntchito litasindikizidwa pa thumba losindikizidwa.
2. Mayesowo akhalebe muthumba lomata mpaka atagwiritsidwa ntchito.
3. Osaundana.
4. Chisamaliro chiyenera kutengedwa kuti chiteteze zigawo za zida kuti zisawonongeke. Osagwiritsa ntchito ngati pali umboni wa kuipitsidwa ndi ma virus kapena mvula. Kuipitsidwa kwachilengedwe kwa zida zogawira, zotengera kapena ma reagents kumatha kubweretsa zotsatira zabodza.

title7

Ganizirani zazinthu zilizonse zochokera kwamunthu ngati zopatsirana ndikuzigwira pogwiritsa ntchito njira zokhazikika zachitetezo chachilengedwe.

Magazi Onse a Capillary
Sambani dzanja la wodwalayo ndikusiya kuti liume. Tsindikani dzanja osakhudza puncture. Phulani khungu ndi lancet wosabala. Chotsani chizindikiro choyamba cha magazi. Pakani dzanjalo pang'onopang'ono kuchokera pamkono kupita pachikhatho kupita ku chala kuti magazi azikhala ozungulira pamalo obowolerapo. Onjezani chitsanzo cha Fingerstick Whole Blood ku chipangizo choyesera pogwiritsa ntchito chubu cha capillary kapena madontho olendewera.

venous Whole Blood:
Sonkhanitsani chitsanzo cha magazi mu chubu chotolera cha lavenda, chabuluu kapena chobiriwira (chokhala ndi EDTA, citrate kapena heparin, motsatana ndi Vacutainer®) popanga mitsempha.

Plasma
Sonkhanitsani chitsanzo cha magazi mu chubu chotolera cha lavenda, chabuluu kapena chobiriwira (chokhala ndi EDTA, citrate kapena heparin, motsatana ndi Vacutainer®) popanga mitsempha. Kulekanitsa plasma ndi centrifugation. Mosamala chotsani madzi a m'magazi mu chubu chatsopano cholembedwapo kale.

Seramu
Sonkhanitsani chitsanzo cha magazi mu chubu chotolera pamwamba chofiyira (chopanda anticoagulants mu Vacutainer®) pobowola mitsempha. Lolani magazi kuti atseke. Kulekanitsa seramu ndi centrifugation. Mosamala chotsani seramu mu chubu chatsopano cholembedwapo kale.
Yesani zitsanzo mwamsanga mutatolera. Sungani zitsanzo pa 2°C-8°C ngati sizinayesedwe msanga.
Sungani zitsanzo pa 2°C-8°C mpaka masiku 5. Zitsanzozi ziyenera kusungidwa pa -20 ° C kuti zisungidwe nthawi yayitali.
Pewani maulendo angapo oundana. Musanayesere, bweretsani zitsanzo zowundana ku kutentha pang'ono ndikusakaniza mofatsa. Zitsanzo zomwe zili ndi zinthu zowoneka bwino ziyenera kufotokozedwa ndi centrifugation musanayesedwe. Osagwiritsa ntchito zitsanzo zowonetsa gross lipemia, gross hemolysis kapena turbidity kuti mupewe kusokoneza pakutanthauzira kwa zotsatira.

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Bweretsani chitsanzo ndi zigawo zoyesera kutentha kozizira Sakanizani chitsanzocho bwino musanachiyese chikasungunuka. Ikani chipangizo choyesera pamalo oyera, ophwanyika.

Kwa zitsanzo zamagazi a capillary:
Kugwiritsa ntchito chubu cha capillary: Dzazani chubu cha capillary ndi kusamutsa pafupifupi 50µL (kapena madontho awiri) a ndodo zonse za chala chitsanzo pachitsime cha chitsanzo (S) cha chipangizo choyesera, kenaka yonjezerani 1 dontho (pafupifupi 30 μL) za Sample Diluent nthawi yomweyo mu chitsime cha chitsanzo. 

Kwa zitsanzo zonse za magazi:
Lembani dropper ndi chitsanzo ndiye tumizani madontho awiri (pafupifupi 50 μL) wa sampuli mu chitsime cha sampuli. Onetsetsani kuti palibe thovu la mpweya. Ndiyetumizani dontho limodzi (pafupifupi 30 μL) ya Sample Diluent yomweyo mu chitsime cha chitsanzo.

Kwa zitsanzo za Plasma/Serum:
Lembani dropper ndi chitsanzo ndiye tumizani dontho limodzi (pafupifupi 25 μL) wa sampuli mu chitsime cha sampuli. Onetsetsani kuti palibe thovu la mpweya. Ndiyetumizani dontho limodzi (pafupifupi 30 μL) ya Sample Diluent yomweyo mu chitsime cha chitsanzo.
Konzani chowerengera. Werengani zotsatira pa mphindi khumi ndi zisanu.Osawerenga zotsatira pambuyo pake 20 mphindi. Pofuna kupewa chisokonezo, taya chipangizo choyesera mutatanthauzira zotsatira

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ZOTSATIRA ZABWINO:
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Gulu limodzi lokha lachikuda limawonekera m'chigawo chowongolera (C). Palibe gulu lowoneka bwino lomwe likuwoneka mdera loyesa (T).

ZOTSATIRA ZOSAVUTA:
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Magulu awiri amitundu amawonekera pa nembanemba. Gulu limodzi limapezeka m'chigawo chowongolera (C) ndipo gulu lina limapezeka m'chigawo choyesera (T).
*ZINDIKIRANI: Kuchuluka kwa mtundu mumzere woyeserera kumasiyana kutengera kuchuluka kwa ma antibodies ku COVID-19 pachitsanzocho. Chifukwa chake, mthunzi uliwonse wamtundu mugawo la mzere woyeserera uyenera kuonedwa kuti ndi woipa.

 

ZOTSATIRA ZAKE:
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Gulu lowongolera likulephera kuwonekera. Zotsatira za mayeso aliwonse omwe sanapange gulu lowongolera pa nthawi yowerengera yotchulidwa ziyenera kunyalanyazidwa. Chonde onaninso ndondomekoyi ndikubwerezanso kuyesa kwatsopano. Vuto likapitilira, siyani kugwiritsa ntchito zidazo nthawi yomweyo ndipo funsani wofalitsa wapafupipafupi.
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1. Ulamuliro Wamkati: Mayesowa ali ndi chowongolera chokhazikika, C band. Mzere wa C umakula mutawonjezera chitsanzo ndi diluent yachitsanzo. Apo ayi, yang'anani ndondomeko yonse ndikubwereza kuyesa ndi chipangizo chatsopano.
2. Ulamuliro Wakunja: Good Laboratory Practice imalimbikitsa kugwiritsa ntchito zowongolera zakunja, zabwino ndi zoyipa (zoperekedwa pakufunsidwa), kutsimikizira kuyesedwa koyenera.


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