COVID-19 Neutralizing Antibody Rapid Test Cassette (Golidi wa Colloidal)
Kaseti ya COVID-19 Neutralizing Antibody Rapid Test (Colloidal Gold) ndi njira yofulumira kwambiri yodziwira ma antibodies ku COVID-19 m'mwazi wathunthu wamunthu, seramu, kapena plasma monga chothandizira pakuzindikira kupezeka kwa ma antibodies. ku COVID-19.
Ma coronaviruses atsopano ndi amtundu wa β.COVID-19 ndi matenda opatsirana pachimake kupuma.Nthawi zambiri anthu amavutika.Pakadali pano, odwala omwe ali ndi kachilombo ka coronavirus ndiye gwero lalikulu la matenda;anthu omwe ali ndi matenda asymptomatic amathanso kukhala gwero lopatsirana.Malingana ndi kafukufuku wamakono wa epidemiological, nthawi yoyamwitsa ndi masiku 1 mpaka 14, makamaka masiku 3 mpaka 7.The mawonetseredwe aakulu monga malungo, kutopa ndi youma chifuwa.Kutsekeka kwa mphuno, mphuno, zilonda zapakhosi, myalgia ndi kutsekula m'mimba zimapezeka nthawi zingapo.
Kaseti ya COVID-19 Neutralizing Antibody Rapid Test (Colloidal Gold) ndi kuyesa kofulumira komwe kumagwiritsa ntchito tinthu tambiri ta S-RBD antigen kuti tipeze ma antibodies omwe amayambitsa COVID-19 m'magazi amunthu, seramu, kapena plasma.
Kaseti ya COVID-19 Neutralizing Antibody Rapid Test (Colloidal Gold) ndi kaseti yoyeserera yodziwikiratu kuti ma antibodies osalowerera ndale ku COVID-19 m'magazi athunthu, seramu kapena plasma.Nembanembayo idakutidwa kale ndi Angiotensin I Converting Enzyme 2 (ACE2) pagawo loyesa la mzere.Poyesedwa, magazi onse, seramu kapena madzi a m'magazi amakhudzidwa ndi S-RBD conjugated colloid golide.Kusakaniza kumasunthira mmwamba pa nembanemba chromatographically ndi capillary kanthu kuti igwirizane ndi ACE2 pa nembanemba ndikupanga mzere wamitundu.Kukhalapo kwa mzere wamtundu uwu kumasonyeza zotsatira zoipa, pamene kusowa kwake kumasonyeza zotsatira zabwino.Kuti ikhale yoyang'anira ndondomeko, mzere wamtundu udzasintha nthawi zonse kuchokera ku Buluu kupita ku Red mu gawo la mzere wolamulira, kusonyeza kuti chiwerengero choyenera cha chitsanzo chawonjezeredwa ndipo kupukuta kwa membrane kwachitika.
| Zipangizo zoyeserera pawokha | Chida chilichonse chimakhala ndi mzere wokhala ndi ma conjugates achikuda ndi ma reagents okhazikika omwe amafalitsidwa kale kumadera ofananirako. |
| Ma pipette otayika | Kuwonjezera zitsanzo ntchito |
| Bafa | Phosphate saline wothira mchere komanso woteteza |
| Ikani phukusi | Pakuti malangizo ntchito |
Zida Zoperekedwa
| ●Zida zoyesera | ●Madontho |
| ●Bafa | ● Ikani phukusi |
Zinthu Zofunika Koma Zosaperekedwa
| ● Zotengera zosonkhanitsira zitsanzo | ● Nthawi |
| ●Chipinda chapakati |
1. Kwa akatswiri mu vitro diagnostic ntchito kokha.
2. Musagwiritse ntchito pambuyo pa tsiku lotha ntchito lomwe lasonyezedwa pa phukusi.Osagwiritsa ntchito mayeso ngati thumba la zojambulazo lawonongeka.Osagwiritsanso ntchito mayeso.
3. The m'zigawo reagent njira lili ndi mchere njira ngati njira kukhudzana khungu kapena diso, kusungunula ndi mvula yambiri madzi.
4. Pewani kuipitsidwa ndi tizitsanzo pogwiritsira ntchito chidebe chosonkhanira chatsopano pa chitsanzo chilichonse chomwe mwapeza.
5. Werengani ndondomeko yonse mosamala musanayesedwe.
6. Osadya, kumwa kapena kusuta m'malo omwe zitsanzo ndi zida zagwiritsiridwa ntchito.Gwirani zitsanzo zonse ngati zili ndi tizilombo toyambitsa matenda.Yang'anirani njira zodzitetezera polimbana ndi zoopsa za microbiological munthawi yonseyi ndikutsata njira zokhazikika zotayira bwino zitsanzo.Valani zovala zodzitchinjiriza monga makhoti a labotale, magolovesi otayika komanso zoteteza maso mukayesedwa.
7. Ngati akuganiziridwa kuti ali ndi kachilombo koyambitsa matenda a coronavirus akuganiziridwa malinga ndi momwe akuwonetsedwera panopo zachipatala komanso miliri zomwe akuluakulu aboma amalimbikitsa, zitsanzo ziyenera kusonkhanitsidwa ndi njira zoyenera zopewera matenda a coronavirus yatsopano ndikutumizidwa ku dipatimenti ya zaumoyo m'boma kapena kwanuko kuti akayezedwe.Chikhalidwe cha ma virus sichiyenera kuyesedwa pamilandu iyi pokhapokha ngati BSL 3+ ilipo kuti mulandire ndi zikhalidwe.
8. Osasinthana kapena kusakaniza ma reagents osiyanasiyana.
9. Chinyezi ndi kutentha kungawononge zotsatira zake.
10. Zida zoyesera zogwiritsidwa ntchito ziyenera kutayidwa malinga ndi malamulo a m'deralo.
1. Zida ziyenera kusungidwa pa 2-30 ° C mpaka tsiku lotha ntchito litasindikizidwa pa thumba losindikizidwa.
2. Mayesowo akhalebe muthumba lomata mpaka atagwiritsidwa ntchito.
3. Osaundana.
4. Chisamaliro chiyenera kutengedwa kuti chiteteze zigawo za zida kuti zisawonongeke.Osagwiritsa ntchito ngati pali umboni wa kuipitsidwa ndi ma virus kapena mvula.Kuipitsidwa kwachilengedwe kwa zida zogawira, zotengera kapena zopangira zida kungayambitse zotsatira zabodza.
Ganizirani zamtundu uliwonse wamunthu ngati zopatsirana ndikuzigwira pogwiritsa ntchito njira zodziwika bwino za biosafety.
Magazi Onse a Capillary
Sambani m'manja mwa wodwala ndipo mulole kuti ziume.Tsindikani dzanja osakhudza puncture.Phulani khungu ndi lancet wosabala.Chotsani chizindikiro choyamba cha magazi.Pakani dzanja pang'onopang'ono kuchokera pamkono kupita pachikhatho kupita ku chala kuti magazi azituluka pamalo oboolapo.Onjezani chitsanzo cha Fingerstick Whole Blood pa chipangizo choyesera pogwiritsa ntchito chubu cha capillary kapena madontho olendewera.
venous Whole Blood:
Sonkhanitsani chitsanzo cha magazi mu chubu chotolera cha lavenda, chabuluu kapena chobiriwira (chokhala ndi EDTA, citrate kapena heparin, motsatana ndi Vacutainer®) popanga mitsempha.
Plasma
Sonkhanitsani chitsanzo cha magazi mu chubu chotolera cha lavenda, chabuluu kapena chobiriwira (chokhala ndi EDTA, citrate kapena heparin, motsatana ndi Vacutainer®) popanga mitsempha.Kulekanitsa plasma ndi centrifugation.Mosamala chotsani plasma mu chubu chatsopano cholembedwapo kale.
Seramu
Sonkhanitsani chitsanzo cha magazi mu chubu chotolera chapamwamba chofiyira (chopanda ma anticoagulants mu Vacutainer®) pobowola mitsempha.Lolani magazi kuti atseke.Kulekanitsa seramu ndi centrifugation.Mosamala chotsani seramu mu chubu chatsopano cholembedwapo kale.
Yesani zitsanzo mwamsanga mutatolera.Sungani zitsanzo pa 2°C-8°C ngati sizinayesedwe msanga.
Sungani zitsanzo pa 2°C-8°C mpaka masiku 5.Zitsanzozi ziyenera kusungidwa pa -20 ° C kuti zisungidwe nthawi yayitali.
Pewani maulendo angapo oundana.Musanayambe kuyezetsa, bweretsani zitsanzo zowundana ku kutentha pang'ono ndikusakaniza mofatsa.Zitsanzo zomwe zili ndi tinthu tating'onoting'ono tating'onoting'ono tikuyenera kufotokozedwa ndi centrifugation musanayesedwe.Osagwiritsa ntchito zitsanzo zosonyeza gross lipemia, gross hemolysis kapena turbidity kuti mupewe kusokonezedwa pakutanthauzira zotsatira.
Bweretsani chitsanzo ndi zigawo zoyesera kutentha kozizira Sakanizani chitsanzocho bwino musanachiyese chikasungunuka.Ikani chipangizo choyesera pamalo oyera, ophwanyika.
Kwa chitsanzo cha magazi a capillary:
Kugwiritsa ntchito chubu cha capillary: Dzazani chubu cha capillary ndikusamutsa pafupifupi 50µL (kapena madontho awiri) a ndodo zonse za chalachitsanzo pachitsime cha chitsanzo (S) cha chipangizo choyesera, kenaka yonjezerani1 dontho (pafupifupi 30 μL)zaSample Diluentnthawi yomweyo mu chitsime cha chitsanzo.
Kwa zitsanzo zonse za magazi:
Lembani dropper ndi chitsanzo ndiyetumizani madontho awiri (pafupifupi 50 μL)wa sampuli mu chitsime cha sampuli.Onetsetsani kuti palibe thovu la mpweya.Ndiyetumizani dontho limodzi (pafupifupi 30 μL)ya Sample Diluent yomweyo mu chitsime cha chitsanzo.
Kwa zitsanzo za Plasma / Serum:
Lembani dropper ndi chitsanzo ndiyekusamutsa dontho limodzi (pafupifupi 25 μL)wa sampuli mu chitsime cha sampuli.Onetsetsani kuti palibe thovu la mpweya.Ndiyetumizani dontho limodzi (pafupifupi 30 μL) ya Sample Diluent yomweyo mu chitsime cha chitsanzo.
Konzani chowerengera nthawi.Werengani zotsatira pa mphindi khumi ndi zisanu.Osawerenga zotsatira pambuyo pake20 mphindi.Pofuna kupewa chisokonezo, taya chipangizo choyesera mutatanthauzira zotsatira
| ZOTSATIRA ZABWINO:
| Gulu limodzi lokha lachikuda limawonekera m'chigawo chowongolera (C).Palibe gulu lowoneka bwino lomwe likuwoneka mdera loyesa (T). |
| ZOTSATIRA ZAKE ZOSAVUTA:
| Magulu awiri amitundu amawonekera pa nembanemba.Gulu limodzi limapezeka m'chigawo chowongolera (C) ndipo gulu lina limapezeka m'chigawo choyesera (T). |
| *ZINDIKIRANI: Kuchuluka kwa mtundu mumzere woyeserera kumasiyana kutengera kuchuluka kwa ma antibodies ku COVID-19 pachitsanzocho.Chifukwa chake, mthunzi uliwonse wamtundu mugawo la mzere woyeserera uyenera kuonedwa kuti ndi woipa. | |
|
ZOTSATIRA ZAKE:
| Gulu lowongolera likulephera kuwonekera.Zotsatira za mayeso aliwonse omwe sanapange gulu lowongolera pa nthawi yowerengera yotchulidwa ziyenera kunyalanyazidwa.Chonde onaninso ndondomekoyi ndikubwereza ndi mayeso atsopano.Vuto likapitilira, siyani kugwiritsa ntchito zidazo nthawi yomweyo ndipo funsani wofalitsa wapafupi. |
1. Ulamuliro Wamkati:Mayesowa ali ndi chowongolera chokhazikika, C band.Mzere wa C umakula mutawonjezera chitsanzo ndi diluent yachitsanzo.Apo ayi, yang'anani ndondomeko yonse ndikubwereza kuyesa ndi chipangizo chatsopano.
2. Ulamuliro Wakunja:Good Laboratory Practice imalimbikitsa kugwiritsa ntchito zowongolera zakunja, zabwino ndi zoyipa (zoperekedwa pakufunsidwa), kutsimikizira kuyesedwa koyenera.
